Table 1.

Cas9 sgRNA spacer sequences and ssDNA donor template sequences used for genomic editing of HepG2 cells

rs2289669 G-to-A substitution
Cas9 sgRNA spacer sequence	5’-CGGGAACTCCCACGCTACTG-3’
ssDNA donor template sequence	5’-AGTTTGTGCTAAGCATCGTAACCTGGGGCTCAGTTTCCACAGTAGCGTGGAAGTTCCCGGCTAGACAAAGGGGATGTTGCAAATCAGTCTTTTCAAAACT-3’
rs2289669 A-to-G substitution
Cas9 sgRNA spacer sequence	5’-CGGGAACTTCCACGCTACTG-3’
ssDNA donor template sequence	5’-AGTTTGTGCTAAGCATCGTAACCTGGGGCTCAGTTTCCACAGTAGCGTGGGAGTTCCCGGCTAGACAAAGGGGATGTTGCAAATCAGTCTTTTCAAAACT-3’
rs8065082 C-to-T substitution
Cas9 sgRNA spacer sequence	5’-TTAGTATCGGCCAAAGTGCC-3’
ssDNA donor template sequence	5’-TATTTTGTTTCCAGTGATAAGAATCCTCTTGGTTTCTTAGTATTGGCCAAAGTGCCCGGTTTGTAGTAGTTGCATAATATGTTT-3’
rs8065082 T-to-C substitution
Cas9 sgRNA spacer sequence	5’-TTAGTATTGGCCAAAGTGCC-3’
ssDNA donor template sequence	5’-TATTTTGTTTCCAGTGATAAGAATCCTCTTGGTTTCTTAGTATCGGCCAAAGTGCCCGGTTTGTAGTAGTTGCATAATATGTTT-3’
rs2289669 deletion
Cas9 sgRNA spacer sequences	5’-GTGCTAAGCATCGTAACCTG-3’
	5’-GAGTTCCCGGCTAGACAAAG-3’
rs8065082 deletion
Cas9 sgRNA spacer sequences	5’-ACCAAGAGGATTCTTATCAC-3’
	5’-TTAGTATCGGCCAAAGTGCC-3’
	5’-TTAGTATTGGCCAAAGTGCC-3’