Figure 2.

CircTRRAP knockdown impeded the damages induced by hypoxia in AC16 cells. AC16 cells were treated with normoxia, hypoxia (24 h), hypoxia+si-NC, or hypoxia+si-circTRRAP. (a) CircTRRAP expression detection was performed using RT-qPCR assay. (b, c) Cell viability (b) and proliferation (c) analysis was performed using CCK-8 and EdU assays, respectively. (d–f) Cell apoptosis assessment was conducted by flow cytometry for apoptotic rate (d), western blot for apoptotic proteins (e), and caspase-3 assay for caspase-3 activity (f). (g–i) Inflammation evaluation was conducted by ELISA for the concentration detection of IL-1β (g), IL-6 (h), and TNF-α (i). (j–l) Oxidative injury analysis was performed by MDA (j), ROS (k), and SOD (l) detection. The experiments were repeated for three times with three paralleled samples. ^∗P < 0.05.