Figure 4.

CircTRRAP/miR-370-3p signal axis regulated the hypoxia-stimulated cell injury. AC16 cells with hypoxic treatment (24 h) were transfected with si-NC, si-circTRRAP, si-circTRRAP+in-miR-NC, or si-circTRRAP+in-miR-370-3p. (a, b) Cell viability (a) and proliferation (b) were assessed via CCK-8 assay and EdU assay. (c–e) Cell apoptosis was measured via flow cytometry (c), western blot (d), and caspase-3 activity assay (e). (f–h) Inflammatory response was analyzed via ELISA. (i–k) Oxidative stress was assessed via the commercial kits. The experiments were repeated for three times with three paralleled samples. ^∗P < 0.05.