Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Feb 9;13:740588. doi: 10.3389/fimmu.2022.740588

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Sarkar, Dhar, Chakraborty, Pati, Bose, Panda, Basak, Chakraborty, Mukherjee, Guin, Jana, Sarkar and Sa

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Tumor-infiltrating Treg cells showed higher expression of CCR4 than the peripheral circulation in breast cancer patients. (A) Representative flow cytometry data of CD4⁺CD25⁺CD127^-FOXP3⁺ Tregs from the peripheral circulation of an age-/sex-matched healthy individual, breast cancer patient, and breast tumor tissue (left panel). In a scatter plot (n = 8; right panel), percent Treg populations from different cohorts were represented. (B) Comparative t-SNE analysis of CCR4⁺ (shown in red/CD4⁺CD25⁺CD127^-CCR4⁺) and CCR4^- (shown in green/CD4⁺CD25⁺CD127^-CCR4^-) Tregs within the proportion of CD4⁺ T cells in the peripheral circulation of an age-/sex-matched healthy individual and a breast cancer patient. (C) Graphical representation of percent CCR4⁺ cells in Th1, Th2, and Treg subpopulations in the peripheral circulation of age-/sex-matched healthy donors and breast cancer patients. (D) Flow cytometric representation of CD4⁺CD25⁺FOXP3⁺CCR4⁺ Tregs in the peripheral circulation of a healthy donor and a breast cancer patient, as well as tumor tissue (left panel). Percentage of CD4⁺CD25⁺FOXP3⁺, CD4⁺CD25⁺FOXP3⁺CCR4⁺, and CD4⁺CD25⁺FOXP3⁺CCR4^- Tregs in the peripheral circulation of age-/sex-matched healthy donor and breast cancer patients, as well as tumor tissue (right panel). (E) Graphical representation of Ki67-mRNA expression in CCR4^- and CCR4⁺ Treg compartments separated from peripheral blood and tumor tissue. As an internal control, GAPDH was used. The values are the mean ± SD of three sets of independent experiments.