Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Feb 9;13:740588. doi: 10.3389/fimmu.2022.740588

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Sarkar, Dhar, Chakraborty, Pati, Bose, Panda, Basak, Chakraborty, Mukherjee, Guin, Jana, Sarkar and Sa

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Cell surface receptor CCR4 interacts with chemokine CCL22 and CCL17 to migrate Treg cells into tumor microenvironment. (A) Relative mRNA expression of CCL22 (upper left panel) and CCL17 (middle left panel) in various macrophage subpopulations (M0, M1, and M2). CCL22 (upper right panel) and CCL17 (middle right panel) relative mRNA expression in neighboring non-tumor/normal tissue and core breast tumor tissue acquired from breast cancer patients at various stages (n = 12). The correlation between CCR4-CCL22 (lower left panel) and CCR4-CCL17 (lower right panel) in core breast tumor tissue taken from various stages of breast cancer patients (n = 12) is depicted in representative correlation coefficient plots. (B) Flow cytometry was used to examine the migration of CD4⁺CD25⁺FOXP3⁺CCR4⁺ Tregs in response to recombinant CCL22 in a trans-well plate (representative plot) (upper panel). Graphical representation of the percentage of CCR4⁺Treg populations after trans-well migration (lower panel). (C) Control-shRNA, CCR4-shRNA, and CCR4-cDNA clones were transfected into ex vivo-generated Treg cells, and intracellular IL10 was measured by flow cytometry (left panel). CCR4 expression was genetically altered in ex vivo-generated Tregs, and percentage cell death was assessed flow cytometrically by Annexin V positivity after 48 h and depicted in a histo-plot (right panel). As an internal control, GAPDH was used. The values are the mean ± SD of three sets of independent experiments.