. 2022 Feb 9;13:814030. doi: 10.3389/fimmu.2022.814030

Table 2.

Comparison of PhenoGraph clusters with manually gated cell phenotypes and associated marker expression.

Cell type	PhenoGraph cluster	Marker expression
Naïve CD4⁺ T cell	PG#4	TCRβ^midCD3^highCD4^highCD62L^highCD44^midLy6c^highCD206^high
Naïve CD4⁺ T cell	PG#17	TCRβ^highCD3^highCD4^highCD62L^highCD44^lowLy6c^midCD206^high
Effector CD4⁺ T cell	PG#11	TCRβ^midCD3^highCD4^highCD62L^lowCD44^highLy6c^midCD69^low
Effector CD4⁺ T cell	PG#23	TCRβ^highCD3^highCD4^highCD62L^midCD44^highLy6c^highCD69^high
Memory CD4⁺ T cell	PG#27	TCRβ^highCD3^highCD4^highCD62L^midCD44^highLy6c^low
Naïve CD8⁺ T cell	PG#13	TCRβ^midCD3^highCD8^highCD62L^highCD44^highLy6c^midCD206^high
Effector CD8⁺ T cell	PG#15	TCRβ^midCD3^highCD8^highCD62L^highCD44^highLy6c^highCD206^high
Memory CD8⁺ T cell	PG#6	TCRβ^lowCD3^highCD8^highCD62L^highCD44^highLy6c^highCD206^mid
B cell	PG#1	B220^highCD19^highCD62L^low CD44^highLy6c^low
	PG#2	B220^highCD19^highCD62L^highCD44^highLy6c^high
	PG#3	B220^highCD19^highCD62L^highCD44^highLy6c^low
	PG#5	B220^highCD19^highCD62L^lowCD44^high Ly6c^high
	PG#12	B220^midCD19^highCD62L^lowCD44^highLy6c^mid
	PG#18	B220^highCD19^highCD62L^highCD44^high Ly6c^highCD11b^lowCD11c^highF4/80^high
	PG#26	B220^highCD19^highCD62L^high CD44^highLy6c^midF4/80^high
	PG#28	B220^highCD19^highNK1.1^highCD62L^midCD44^highLy6c^mid
	PG#29	B220^highCD19^highCD62L^midGr-1Ly6c^mid
NK cell	PG#8	NK1.1^highCD11c^lowCD11b^midLy6c^high
NK cell	PG#10	NK1.1^highCD11c^midCD11b^lowLy6c^low
Monocyte	PG#7	CD11b^highCD11c^lowGr-1^lowLy6c^highCD44^high
	PG#20	CD11b^highCD11c^lowLy6c^highF4/80^highCD206^low
	PG#22	CD11b^highCD11c^lowLy6c^highF4/80^highCD206^low
Dendritic cell	PG#9	CD11b^highCD11c^highLy6c^highCD44^high
Granulocyte	PG#24	CD11b^highCD11c^lowLy6c^highGr-1^highF4/80^lowCD206^low
Unassigned	PG#14	TCRβ^lowCD3^highCD4^highCD62L^highCD44^highLy6c^highCD206^high
	PG#16	B220^highCD19^highCD62L^midCD44^highLy6c^highCD69^high
	PG#19	TCRβ^highCD3^highCD8^highCD62L^highCD44^highLy6c^high
	PG#21	CD11c^highCD11b^lowLy6c^highF4/80^lowCD206^low
	PG#25	B220^highCD19^highNK1.1^highCD62L^high CD44^highLy6c^highGr-1^midCD206^highCD25^high