. 2022 Feb 9;13:814030. doi: 10.3389/fimmu.2022.814030

Table 3.

Comparison of FlowSOM clusters with manually gated cell phenotypes and associated marker expression.

Cell type	FlowSOM cluster	Marker expression
Naïve CD4⁺ T cell	FS14	TCRβ^highCD3^highCD4^highCD62L^midCD44^midLy6c^midCD206^high
Effector CD4⁺ T cell	FS16	TCRβ^highCD3^highCD4^highCD62L^midCD44^highLy6c^midCD69^high
Effector CD4⁺ T cell	FS20	TCRβ^highCD3^highCD4^highCD62L^lowCD44^highLy6c^low
Memory CD4⁺ T cell	FS7	TCRβ^highCD3^highCD4^highCD8^midCD62L^highCD44^highLy6c^highCD206^high
Naïve CD8⁺ T cell	FlS11	TCRβ^lowCD3^highCD8^highCD62L^lowCD44^midLy6c^lowCD69^highCD206^high
Effector CD8⁺ T cell	FS8	TCRβ^midCD3^highCD8^highCD62L^highCD44^lowLy6c^lowGr-1^highCD206^mid
Memory CD8⁺ T cell	FS5	TCRβ^midCD3^highCD8^highCD62L^highCD44^highLy6c^highCD206^mid
B cell	FS9	B220^highCD19^highCD62L^mid CD44^highLy6c^low
	FS12	B220^highCD19^highCD62L^low CD44^highLy6c^lowCD69^high
	FS17	B220^highCD19^highCD62L^low CD44^highLy6c^low
	FS18	B220^highCD19^midCD62L^mid CD44^highLy6c^high
NK cell	FS6	NK1.1^highCD11c^lowCD11b^lowLy6c^high
NK cell and DC	FS1	NK1.1^highCD11c^midCD11b^midLy6c^low
Monocyte	FS2	CD11b^highCD11c^midLy6c^lowF4/80^highCD206^high
Monocyte	FS3	CD11b^highCD11c^midLy6c^highF4/80^lowCD206^low
Granulocyte	FS4	CD11b^highCD11c^lowLy6c^highGr-1^highF4/80^lowCD206^low
Unassigned	FS10	TCRβ^midCD3^highCD4^highCD8^highCD62L^highCD44^highLy6c^highCD206^midCD69^high
	FS13	TCRβ^lowCD3^highCD8^highCD62L^lowCD44^highLy6c^lowCD69^highCD206^high
	FS15	TCRβ^-CD3^highCD4^highCD8^highCD62L^lowCD44^highLy6c^lowCD69^highCD206^midCD25^high
	FS19	TCRβ^highCD3^highCD4^highCD8^highCD62L^lowCD44^highCD206^high