FIGURE 1.

CHIKV infection induces the expression of both subunits of IL27 and activated a dynamic transcriptional profile in human MDMs. Human MDMs cultures were left uninfected or infected with CHIKV at MOI 5. Culture supernatants were obtained at 6, 24, and 48 hpi, and quantification of CHIKV PFU/mL was performed by plaque assay on Vero cells. MDMs lysates were obtained at 6, 24, and 48 hpi, and RT-qPCR was performed. CHIKV replication, IL27p28 and EBI3 mRNA expression kinetics in human MDMs (A). Data are presented as the mean ± SEM. Mann-Whitney test was performed. Significant results are defined as p < 0.05 (*), p < 0.01 (**) and p < 0.002 (***). n = 4. Pearson’s correlation between CHIKV replication (PFU/ml) and IL27p28 mRNA expression (Log2 FC) in CHIKV-infected MDMs (A). Significant results are defined as p < 0.05 (*), p < 0.01 (**) and p < 0.002 (***). Human MDMs cultures were left uninfected or infected with CHIKV at MOI 5. Cell lysates were obtained at 6 and 24 hpi and RNA-seq was performed. PCA plot (B) of differentially expressed genes (DEG) interrogated by RNA-Seq. MA plots (C and E) and GO enrichment analysis (D and F) from DEG regulated in CHIKV-infected MDMs at 6 and 24 hpi, respectively. DEG in CHIKV-infected MDMs was selected from genes with an FDR< 0.05 and |Log2 Fold Change (CHIKV-infected MDMs/Uninfected MDMs) |> 0.6. To quantify mRNAs abundance, we use the Reads per kilobase per million mapped reads (RPKM). n = 4.