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. 2022 Feb 9;10:812110. doi: 10.3389/fcell.2022.812110

FIGURE 2.

FIGURE 2

The human encoding genes for IL27p28 and EBI3 have differential transcriptional regulation. TFBM analysis was performed on promoters (−600 and +50 relative to TSS) of human IL27p28 and EBI3 genes using HOMER software. TFBM on the promoter of human IL27p28 and EBI3 genes (A). Human MDMs cultures were left uninfected or infected with CHIKV at MOI 5. Cell lysates were obtained at 6 and 24 hpi and RNA-seq was performed. mRNAs abundance (RPKM) of IL27 subunits (IL27p28 and EBI3) and transcription factors (IRF1, IRF7 and NF-κB1) in uninfected and CHIKV-infected MDMs (B) Data are presented as the mean ± SEM. Kruskal–Wallis test with Dunn’s post-test was performed. Significant results between uninfected and CHIKV-infected MDMs are defined as p < 0.05 (*), p < 0.01 (**) and p < 0.002 (***). n = 4. Pearson’s correlation between transcription factors (IRF1, IRF7 and NF-κB1) and IL27 subunits (IL27p28 and EBI3) mRNAs abundance (RPKM) in uninfected and CHIKV-infected MDMs (C). Significant results are defined as p < 0.05 (*), p < 0.01 (**) and p < 0.002 (***).