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. 2022 Feb 9;10:812110. doi: 10.3389/fcell.2022.812110

FIGURE 3.

FIGURE 3

TLR4 activation in human MDMs triggers NF-κB-complex activation which drives the NF-κB-target genes expression. We reanalyzed a publicly available RNA-seq dataset [(GSE84188 (GEO) (Olejnik et al., 2017)], obtained from human MDMs stimulated with 100 ng/ml of LPS, for 6, 24 or 48 h. MA plot of DEG (A) and GO enrichment analysis (B) from DEG regulated in LPS-stimulated MDMs at 6 h and. DEG in LPS-stimulated MDMs was selected from genes with an FDR< 0.05 and |Log2 Fold Change (LPS-stimulated MDMs/unstimulated MDMs |> 0.6. To quantify mRNAs abundance, we use the Reads per kilobase per million mapped reads (RPKM). n = 3. mRNAs abundance (RPKM) of TLR4 signaling pathway components (C), NF-κB-complex components (D), and NF-κB-target genes (E) in unstimulated and LPS-stimulated MDMs. Data are presented as the mean ± SEM. Kruskal–Wallis test with Dunn’s post-test was performed. Significant results between unstimulated and LPS-stimulated MDMs are defined as p < 0.05 (*), p < 0.01 (**) and p < 0.002 (***). Significant results between treatment-times comparisons are defined as p < 0.05 (#), p < 0.01 (##) and p < 0.002 (###).