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. 2022 Feb 9;10:812110. doi: 10.3389/fcell.2022.812110

FIGURE 5.

FIGURE 5

The CHIKV infection of MDMs induces the mRNA expression of TLR3 signaling components, IRF1, and IL27p28. Human MDMs cultures were left uninfected or infected with CHIKV at MOI 5. Cell lysates were obtained at 6 and 24 hpi, and RNA-seq and RT-qPCR were performed. mRNAs abundance (RPKM) of TLR signaling pathway components and IRFs (A), IFNs and IL27p28 (B), in uninfected and CHIKV-infected MDMs. Data are presented as the mean ± SEM. Kruskal–Wallis test with Dunn’s post-test was performed. Significant results between uninfected and CHIKV-infected MDMs are defined as p < 0.05 (*), p < 0.01 (**) and p < 0.002 (***). Significant results between treatment-times comparisons are defined as p < 0.05 (#), p < 0.01 (##) and p < 0.002 (###). n = 4. Pearson’s correlation between TLR3 (C) or TLR7 (D), with IL27p28 mRNA abundance (RPKM), in uninfected and CHIKV-infected MDMs. Significant results are defined as p < 0.05 (*), p < 0.01 (**) and p < 0.002 (***). Human MDMs cultures were infected at MOI 5 of CHIKV or a non-replicative UV-inactivated CHIKV (UV-CHIKV). CHIKV replication (E) was measure by plaque assay on vero cells at 0, 6, 24 and 48 hpi. TLR3 mRNA expression (Log2 FC) (F) and IL27 cytokine production (G) were evaluated at 0, 6 and 24 hpi, by RT-qPCR and ELISA, respectively. Data are presented as the mean ± SEM. Kruskal–Wallis test with Dunn’s post-test was performed. Significant results between uninfected and CHIKV- or UV-CHIKV-infected MDMs are defined as p < 0.05 (*), p < 0.01 (**) and p < 0.002 (***). Significant results between CHIKV-infected MDMs and UV-CHIKV-infected MDMs are defined as p < 0.05 (#), p < 0.01 (##) and p < 0.002 (###). n = 4.