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. 2022 Jan 19;27(1-2):112–132. doi: 10.1007/s10495-021-01706-9

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Fig. 7 — Overexpression of hUsp27x_L does reduce ubiquitination of TRIM28 but not of RIPK1. TetR-GFP-hUsp27x_L WM1158 cells overexpressing His-ubiquitin were treated as indicated, lysed and Ni²⁺-NTA agarose beads were used to pull down His-ubiquitin labelled proteins (HisUbi-pulldown). Purified proteins were loaded alongside input samples (whole-cell-lysate) on SDS-gels to perform Western blot or Coomassie-stain. Data show one representative experiment (n = 3)