Table 1.
qRT-PCR primers sequences
| Gene/symbol | Forward sequence | Reverse sequence |
|---|---|---|
| GLI1 | AGCCTTCAGCAATGCCAGTGAC | GTCAGGACCATGCACTGTCTTG |
| GLI2 | CTGTGGGTTAGGGATGGACTG | GTAAAGTGGGTGGACGTTGCA |
| GLI3 | GTGCTCCACTCGAACAGA | TCCAGGACTTTCATCCTCATTAGA |
| SMO | TGAAGGCTGCACGAATGAGG | CTTGGGGTTGTCTGTCCGAA |
| BCL-2 | TGTGTGTGGAGAGCGTCAAC | GCCAGAGAAATCAAACAGAGG |
| CCND1 | CTTCAAATGTGTGCAGAAGG | CTCGCACTTCTGTTCCTC |
| P21 | CCCTTGTCCTTTCCCTTCAGTAC | GTGGGACAGGCACCTCAGA |
| BMP4 | CGTAGCCCTAAGCATCACTCACA | GCGCCGGCAGTTCTTATTCT |
| FoxM1 | GGGCGCACGGCGGAAGATGAA | CCACTCTTCCAAGGGAGGGCTC |
| c-MYC | AATGAAAAGGCCCCCAAGGTAGTTATCC | GTCGTTTCCGCAACAAGTCCTCTTC |
| P27 | ATGTCAAACGTGCGAGTGTCT | TTACGTTTGACGTCTTCTGA |
| VEGF | CTACCTCCACCATGCCAAGT | GCAGTAGCTGCGCTGATAGA |
| TP53 | TGTAGTGGATGGTGGTACAG | CGTGTGGAGTATTTGGATGAC |
| B2M | CAGCCCAAGATAGTTAAGTG | CCCTCCTAGAGCTACCTGT |