Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Feb 22;13:998. doi: 10.1038/s41467-022-28493-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a The strategy to label mPFC-SI projection neurons and mPFC-SUM projection neurons with CTb. b, c The injection site of CTb in SI and SUM. d An example image showed the distribution of CTb labeled neurons in the mPFC. e Quantification of the distribution of different CTb labeled neurons in mPFC along the depth of the cortical layers. n = 7 animals. f Quantification of the percentage of different CTb labeled neurons in the mPFC, n = 7 animals. one-way repeated-measures (RM) ANOVA with Tukey’s post hoc test. SI projecting vs double projecting, p = 3.1×10^-14; SUM projecting vs double projecting, p = 2.7×10^-14. g The experimental strategy of sparse labeling of the morphology of PFC-SI projection neurons and PFC-SUM projection neurons with AAV. h An example image showed the sparse labeled neurons in the mPFC. i Quantification of the dendritic length of different types of neurons. one-way repeated-measures (RM) ANOVA with Tukey’s post hoc test, CT neurons, n = 6; IT neurons, n = 6; PT neurons, n = 4. j The whole-brain axon projection of different types of neurons in the mPFC and their main target brain areas. Scale bars in b, c are 500 μm. Scale bar in d is 100 μm. Scale bar in h is 100 μm. A anterior, D dorsal. L, lateral, M medial, P posterior, V ventral, SI substantia innominata, SUM supramammillary nucleus, IT intratelencephalic, PT pyramidal tract, CT corticothalamic, ACA anterior cingulate area, ORB orbital cortex, AON anterior olfactory nucleus, CP, caudate putamen, ACB nucleus accumbens, PVT paraventricular thalamic nucleus, AM anteromedial thalamic nucleus, MD mediodorsal thalamic nucleus, RE reuniens thalamic nucleus, LHA lateral hypothalamic area, DMH dorsomedial hypothalamic nucleus, VTA ventral tegmental area, SNR substantia nigra, reticular part. All data are listed as the Mean ± SEM.