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. 2022 Feb 22;13:998. doi: 10.1038/s41467-022-28493-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Expressing the acetylcholine indicators in Fezf2 positive neurons in the mPFC with a Cre dependent virus. The Cre expression was induced by tamoxifen 3 days after the virus injection. b The scheme of fiber photometry. c The expression of acetylcholine indicators in Fezf2 positive neurons in the mPFC. d The experimental design of object recognition test. e Heatmap of acetylcholine response of extratelencephalic projection neurons in the PL of Fezf2-CreER mice during object interactions. f Average plots across different trials of individual animals of acetylcholine response of extratelencephalic projection neurons in PL of Fezf2-CreER mice during object interactions. g Heatmap of acetylcholine response of extratelencephalic projection neurons in PL of AD_Fezf2-CreER mice during object interactions. h Average plots across different trials of individual animals of acetylcholine response of extratelencephalic projection neurons in PL of AD_Fezf2-CreER mice during object interactions. i Average plots of acetylcholine response of extratelencephalic projection neurons in the PL of both Fezf2-CreER mice and AD_Fezf2-CreER mice during multiple tests on multiple animals (Fezf2-CreER, n = 12 animals; AD_Fezf2-CreER mice, n = 9 animals). j The quantification of AUC in i The acetylcholine response of extratelencephalic projection neurons in PL of both Fezf2-CreER mice is stronger than that in AD_Fezf2-CreER mice. (Two-tailed Mann-Whitney test, Fezf2-CreER mice, n = 12 animals; AD_Fezf2-CreER mice, n = 9 animals.) Scale bar in c is 200 μm. ACA anterior cingulate area, PL prelimbic area, TM tamoxifen. All data are listed as the Mean ± SEM. The color bars in the figure indicated df/f (%).