Fig. 7. Activation of cholinergic axon terminals in the mPFC of 5×FAD mice improved object recognition memory expression.
a The experimental strategy of activation of cholinergic terminals in the mPFC. b Immunostaining against ChAT to validate the specificity of the AAV. c Heat-map plots showed object recognition memory measures from the object recognition test. Red = more time, blue = less time. The heatmap in the left and right showed examples of ADChAT-Cre mice that expressed ChR2 in the cholinergic terminals in the mPFC exploring the familiar object and novel object during test session without or with light stimulation, respectively. d Statistical plots of object recognition index and delta object recognition index in laser off and laser on sessions. (left and right, two-tailed paired t test). n = 5 animals. e The statistical plot showed the traveling distance of ADChAT-Cre mice with or without light activation of the cholinergic terminals in the mPFC. two-tailed paired t test, n = 5 animals. f The heatmap in the left and right showed examples of ADChAT-Cre mice that expressed GFP in the cholinergic terminals in the mPFC exploring the familiar object and novel object during test session without or with light stimulation, respectively. g Statistical plots of object recognition index and delta object recognition index in laser off and laser on sessions. (left and right, two-tailed paired t test). n = 5 animals. h Statistical plots showed the traveling distance in GFP group with or without light stimulation. two-tailed paired t test, n = 5 animals. Scale bar in b is 50 μm. All data are listed as the Mean ± SEM.