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. 2022 Feb 22;5:153. doi: 10.1038/s42003-022-03108-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — 4-week-old male wild type and Hjv^−/− mice (n = 8–10 per group) were fed a standard diet, a high iron diet (HID) or an iron-deficient diet (IDD). All animals were sacrificed after 5 weeks; livers were dissected and processed for biochemical, histological and proteomics analysis. a Schematic representation of the experimental design. b qPCR analysis of Hamp mRNA. c Quantification of liver iron. d Perls Prussian blue staining in liver sections (magnification: 3x). Data in (b–c) are presented as the mean ± SEM. Statistical analysis was performed by two-way ANOVA. Statistically significant differences across genotypes are indicated by **(p < 0.01) or ***(p < 0.001).