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. 2022 Feb 22;5:153. doi: 10.1038/s42003-022-03108-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — Primary hepatocytes were isolated from wild type and Hjv^−/− mice and left untreated or treated overnight with 15 μg/ml ferric ammonium citrate (FAC) or 100 μM desferrioxamine (DFO), respectively (n = 7–8 technical replicates for each condition). Subsequently, the cells were analyzed for oxygen consumption rate (OCR) using the Seahorse assay. a OCR following addition of oligomycin, FCCP and rotenone/antimycin A. b OCR for basal respiration. c OCR for spare respiratory capacity. d OCR for proton leak. e OCR for ATP production. f OCR for maximal respiratory capacity. Data are presented as the mean ± SEM. Statistical analysis was performed by one-way ANOVA. Statistically significant differences are indicated by *(p < 0.05).