Skip to main content
. 2022 Feb 22;8:77. doi: 10.1038/s41420-022-00850-8

Fig. 1. BMSCs are identified and induced to differentiate to HLCs by HGF.

Fig. 1

A Microscopic observation of cell morphology of BMSCs. B Flow cytometry analysis for CD105, CD34, and CD45 expression to identify BMSCs. C RT-qPCR to detect the expression of ALB in NCTC 1469 hepatocytes, HGF-treated BMSC, and untreated BMSCs. D RT-qPCR to detect the expression of CK18 in NCTC 1469 hepatocytes, HGF-treated BMSC, and untreated BMSCs. E RT-qPCR to detect the expression of TTR in NCTC 1469 hepatocytes, HGF-treated BMSC, and untreated BMSCs. F RT-qPCR to detect the expression of AFP in NCTC 1469 hepatocytes, HGF-treated BMSC, and untreated BMSCs. G ELISA to determine the content of ALB in the culture supernatant of NCTC 1469 hepatocytes, HGF-treated BMSC, and untreated BMSCs. H Glycogen content in NCTC 1469 hepatocytes, HGF-treated BMSC, and untreated BMSCs measured by PAS staining. I The expression of the BMSC marker CD105 and hepatocyte markers AFP and ALB in NCTC 1469 hepatocytes, HGF-treated BMSC, and untreated BMSCs, as detected with immunofluorescence staining (CD105/AFP/ALB in green, DAPI-stained nuclei in blue). *p < 0.05 vs. NCTC 1469 cells; #p < 0.05 vs. untreated BMSCs. The cell experiments were repeated three times.