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. 2022 Feb 22;8:77. doi: 10.1038/s41420-022-00850-8

Fig. 6. LncRNA SNHG1 silencing orchestrates the miR-15a/SMURF1 axis to induce HLC differentiation of mouse BMSCs.

Fig. 6

A RT-qPCR to detect hepatocyte-related gene expression in differentiated BMSCs after silencing of lncRNA SNHG1 or/and inhibition of miR-15a for 14 days. B ELISA to detect ALB content in culture supernatant of BMSCs after silencing of lncRNA SNHG1 or/and inhibition of miR-15a for 14 days. C The glycogen accumulation in BMSCs after silencing of lncRNA SNHG1 or/and inhibition of miR-15a for 14 days determined by PAS staining. D RT-qPCR to assess hepatocyte-related gene expression in differentiated BMSCs after overexpressing miR-15a or/and overexpressing SMURF1 for 14 days. E ELISA to assess ALB content in culture supernatant of BMSCs after overexpressing miR-15a or/and overexpressing SMURF1 for 14 days. F Glycogen accumulation in BMSCs after overexpression of miR-15a or/and overexpression of SMURF1 for 14 days evaluated by PAS staining. *p < 0.05 vs. BMSCs transfected with sh-NC + inhibitor NC/NC mimic + oe-NC; #p < 0.05 vs. BMSCs transfected with sh-SNHG1 + inhibitor NC/miR-15a mimic + oe-NC. The cell experiments were repeated three times.