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. 2022 Feb 22;13(2):171. doi: 10.1038/s41419-022-04627-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Gel images for validated eccDNAs amplified with no gene ([chr5^{circle 151033-151034 kb}]). B eccDNA amplified with multiple coding genes ([chr1^{circle 46219-52682 kb}]). C eccDNA ([ROR2^{circle exon 1-5}] and (D) [ROR2^{circle intron 4}]) by outward PCR (blue arrows), inward PCR (black arrows), gel electrophoresis, schematic diagram and Sanger sequencing. EccDNAs are named according to gene content. blue boxes: exons. M: Marker. F: eccDNA of FaDu cells. D: eccDNA of FaDu/DDP cells. FG: genomic DNA of FaDu cells. DG: genomic DNA of FaDu/DDP cells. φF: phi29 (φ) amplified eccDNA of FaDu cells. φD: phi29 (φ) amplified eccDNA of FaDu/DDP cells. NTC: nontemplate control.