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. 2022 Feb 22;13:863. doi: 10.1038/s41467-022-28426-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Comparison (dots) of the relative abundances of cOTUs between a pair of technical replicates (other pairs in Supplementary Fig. 12). Orange lines, theoretical confidential interval (99.9%) for sampling noise based on the Poisson distribution and normalization to the relative abundance (to the proportional abundance for b and c); cyan lines, 2-fold change; magenta lines, 10-fold change; blue dots, cOTUs that showed larger difference than that of the sampling noise and 2-fold change and was smaller than the 10-fold change; proportions and numbers in the parentheses, proportions and the numbers of dots in corresponding colors, respectively; CEa, mouse; dist and prox, locations; 1 and 2, technical replicates (see text). b Comparison between the proportional abundances of the common pairs of cOTUs and ASVs with all 16 cell-samples shown in the same format as a. Red dots, common pairs of cOTUs and ASVs that showed larger difference than the sampling noise and 10-fold change. c Comparison between the proportional abundances of the common pairs of cOTUs and OTUs with all 16 cell-samples shown in the same format as a and b. Source data are provided as a Source Data file.