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. 2022 Feb 22;13:863. doi: 10.1038/s41467-022-28426-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a The cOTU richness of each cell-sample determined by subsampling 6608 cells using the function rarefy in the R package Vegan. CE2, CE2 nutriment group; VA-suf, VA-sufficient group; VA-def, VA-deficient group; dist and prox, locations. b, d, and f, Principal coordinate analysis (PCoA) of Bray-Curtis dissimilarities calculated based on the relative cell abundances of cOTUs (b), ASVs (d), and OTUs (f) between each pair of cell-samples in the VA group. Labels, same as (a); gray line, linkage from the same mouse; circles, 95% confidence ellipses for each group. c, e, and g, Quantitative comparison of Bray-Curtis dissimilarities in b, d, and f, respectively. Distal, all possible pairs from VA-suf^dist and VA-def^dist, respectively; Proximal, all possible pairs from VA-suf^prox and VA-def^prox, respectively. Boxes in a, c, e, and g represent 25th to 75th percentiles (the interquartile range), horizontal black lines indicate medians, and whiskers show 1.5 times the interquartile range (n = 3 for CE2^dist and CE2^prox; n = 4 for VA-suf^dist, VA-suf^prox, VA-def^dist, and VA-def^prox; n = 16 for Distal and Proximal). P values were calculated by the Kruskal–Wallis rank-sum test. Source data are provided as a Source Data file.