TABLE 2.
Summary table of correlation between resistome and ATBs in concentrations corresponding to the three ranges of the MSW hypothesis.
| Antibiotic concentration/Related RGs | UPS |
DWS |
||||
| < MSC | MSC-MIC | > MIC | < MSC | MSC-MIC | > MIC | |
| CPR | – | – | – | – | + | + |
| – | – | – | – | qnrA | qnrA | |
| ENR | + | – | + | + | – | + |
| qnrA, qnrS | – | qnrB, qnrC, mfsA | qnrA, qnrS, mfsA | – | qnrA, qnrS, mfsA | |
| FMQ | + | + | – | – | + | – |
| – | qnrB, qnrC, mfsA | – | – | qnrS | – | |
| LVX | – | + | + | – | + | + |
| – | qnrA, qnrS | qnrA, qnrS | – | qnrA | qnrA | |
| NFX | + | + | + | – | + | + |
| – | mfsA | – | qnrA | qnrA, mfsA | ||
| AZM | – | + | + | – | + | + |
| – | ermC, ermX, macB, mefA_10 | ermC, mefA_10 | – | ermC | ermC | |
| CLR | + | + | – | – | + | + |
| – | macB, mefA_10, ermC | – | – | – | – | |
| ERY | + | – | – | – | + | + |
| ermX, macB | – | – | – | – | – | |
| RXM | + | – | – | + | – | + |
| ermC, ermX, macB, mefA_10 | – | – | mefA_10 | – | – | |
| SPR | + | + | – | – | + | + |
| – | – | – | – | ermC, ermX, ermY, macB | – | |
| T-T | + | – | – | + | – | – |
| ermC, ermX, macB, mefA_10 | – | – | – | – | – | |
| SMX | + | – | – | + | – | – |
| sul1, sulA | – | – | sul1, sulA | – | – | |
| MTZ | + | + | – | – | + | – |
| – | – | – | – | – | – | |
| OTC | + | + | – | + | – | – |
| – | – | – | tetB, tetQ | – | – | |
| TMP | + | + | + | + | + | + |
| – | – | – | – | – | – | |
The first column lists all antibiotics with already defined concentration ranges for the mutant selection window (MSC and MIC). There are two rows opposite each antibiotic. The top row shows the concentration range in which the antibiotic was measured in the biofilm (highlighted in green), and the bottom row shows whether the network analysis showed a link between that antibiotic and the corresponding RGs, and if so, with which gene.