Fig. 2.

M2-Microglia CM promotes NSCLC cells colonization. a, b E-cadherin (magnification: 400×) and vimentin (magnification: 200×) expression of A549 and H292 cells were presented by immunofluorescence staining. The blue signal represents the DAPI-stained nuclei. c qRT-PCR analysis of E-cadherin and vimentin for A549 cells cultured with different treatment. d qRT-PCR analysis of E-cadherin and vimentin for H292 cells cultured with different treatment. e Western blot for E-cadherin and vimentin of A549 cells cultured under different conditions. The right panels were gray value analysis. f Western blot for E-cadherin and vimentin of H292 cells cultured under different conditions. The right panels were gray value analysis. control: normal A549 cells; TGF-β1: A549 cells treated with TGF-β1 (2.5 ng/ml) for 24 h; co-HCM and co-CCM: A549 cells pretreated with TGF-β1 (2.5 ng/ml) and incubated with the co-cultured CM for 24 h. co-HCM/co-CCM: CM from A549 and HMO6/CHME5 cells co-cultured for 48 h. Data are mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001