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. 2022 Feb 23;7:52. doi: 10.1038/s41392-022-00872-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — IL6 emerged as for a mediator of A549-F3 and M2 microglia communication in BM. a Heat map showed different gene expression profiles of A549-F3 populations and parental cells. b Heat map showed different gene expression profiles of A549 co-cultured with HMO6 for 48 h and parental cells. c Venn diagram of genes positively correlated with brain metastasis analyzed through transcriptome microarrays between parent A549 cells and A549-F3 or co-cultured A549 cells. d Comparison of mRNA microarray data showed top 30 changed genes (up or down) with the same expressed pattern between parent A549 cells and A549-F3 or co-cultured A549 cells. e Heat map of the most prominently upregulated cytokine in A549-F3 cells and co-cultured system. f qRT-PCR analysis showed expression of IL6 in different cell lines. g Western blot showed expression of IL6 in different cell lines. h ELISA assay of IL6 in culture supernatants from A549 and A549-F3 cells. i Brain metastases were determined by bioluminescence imaging. j Histogram showed incidence of brain metastases in different cell lines. k Photon flux detected from brain-metastatic focus in the different groups. Data are mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001