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. 2022 Feb 9;9:770017. doi: 10.3389/fmed.2022.770017

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Copyright © 2022 Amorim, McGovern, Raposo, Khatiwada, Shen, Koentgen, Hold, Behary, El-Omar and Zekry.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Antibiotic treatment reduced bacterial content of mouse gut after three days. (A) Bacterial culture of stool samples on 10% horse blood agar plates; (B) DNA concentration (ng/mg of stool) of stool samples during the 21-day course of antibiotic treatment with ampicillin, vancomycin, neomycin and metronidazole (****p < 0.0001, One-way ANOVA); (C) Variation in Ct value of 16S rRNA gene amplification during antibiotic treatment (****p < 0.0001, One-way ANOVA). Positive and negative controls for 16S are represented in the graph; (D) Representative pictures of fluorescent in-situ hybridisation at day 0 (D0; left panel), day 3 (D3; middle panel) and day 7 (D7; right panel) of antibiotic treatment. Scale bar 2 μm.