FIGURE 2.

Anti-ASC inhibits NLRP1 inflammasome activation in the cortex of aged mice. Mice were treated with anti-ASC (10 mg/kg) and saline control (i.p.) and sacrificed 3 days later. (A) Representative immunoblot of cortical protein lysates of young (3 months), aged (18 months) and anti-ASC-treated aged mice (mAb) blotted for NLRP1 (B), caspase-1 (C), ASC (D), and ASC specks (E). Data presented as mean ± SEM. 3 m, 3 months; Sal, Saline; mAb, Monoclonal antibody. N = 6 per group. *p < 0.05. β-actin was used as a protein loading control and internal standard.