Figure 4.

Osteogenesis network construction and cell type-specific marker immunofluorescence analysis of developing TLV and RP

A. Module visualization of the network connections and associated functions using MATN1 as a hub gene. Gene-connected intra-modular degree is simultaneously indicated by spot size and color intensity. The hub gene MATN1 is indicated in yellow. B. and C. Immunofluorescence analysis of MATN1 and HMGB2 in TV and RP (B) and in LV (C). Red and green indicate fluorescence signals of MATN1 and HMGB2, respectively. White and yellow triangles indicate MATN1⁺ and HMGB2⁺ cells, respectively. D. and E. Immunofluorescence analysis of COL1A1 in TV and RP (D) and in LV (E). Red indicates fluorescence signals of COL1A1. Yellow triangles indicate COL1A1⁺ cells. White, blue, and red dashed lines in (B–E) indicate regions of TV, LV, and RP, respectively. Scale bar, 400 μm.