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. 2021 Nov 11;19(3):423–436. doi: 10.1016/j.gpb.2021.09.008

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Angiogenesis network construction and cell type-specific marker immunofluorescence analysis of developing TLV and RP

A. Module visualization of the network connections and associated functions using CD34 as a hub gene. Gene-connected intra-modular degree is simultaneously indicated by spot size and color intensity. The hub gene CD34 is indicated in yellow. B. and C. Immunofluorescence analysis of CD93, CD248, and CD34 in TV and RP (B) and in LV (C). Green, white, and red indicate fluorescence signals of CD93, CD248, and CD34, respectively. White, yellow, and blue triangles indicate CD93⁺, CD248⁺, and CD34⁺ cells. White, blue, and red dashed lines in (B and C) indicate the regions of TV, LV, and RP, respectively. Scale bar, 400 μm.