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. 2022 Feb 9;10:755260. doi: 10.3389/fbioe.2022.755260

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Copyright © 2022 Zhu, Ye, Cai, Li, Fan and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Intra-articular injection of BMSCs incorporating PHa@I hydrogels enhance chondrogenesis. (A–D) RT-qPCR analysis of chondrogenic-related genes Sox 9, Col-2a1, Aggrecan, and HIF-1α in cartilage at 12 weeks post-injection. (E) Representative double immunofluorescence images of Sox 9, Col-2a1, Aggrecan, and HIF-1α in cartilage at 12 weeks post-injection. (F–I) Quantitative analysis of fluorescence intensity of Sox 9, Col-2a1, Aggrecan, and HIF-1α in immunofluorescence staining (*p < .05, **p < .01, and ***p < .001 compared with the PBS Group; ^# p< .05 and ^## p < .01 compared with the PHa@I Group; ^& p < .05 compared with the BMSC Group, n = 6).