TABLE 1.
Primer sequences of genes.
| mRNA | Oligonucleotide primers (5′–3′) | NCBI reference sequence |
|---|---|---|
| GAPDH | F-ATGGGAAGCTGGTCATCAAC | NCBI Reference Sequence: NM_017008.4 |
| R-GGATGCAGGGATGATGTTCT | ||
| Sox 9 | F-AGGAAGCTGGCAGACCAGTA | NCBI Reference Sequence: NM_080403.2 |
| R-CGGCAGGTATTGGTCAAACT | ||
| Col-2a1 | F-CGAGGTGACAAAGGAGAAGC | NCBI Reference Sequence: NM_012929.1 |
| R-CTGGTTGTTCAGCGACTTGA | ||
| Aggrecan | F-TGGCATTGAGGACAGCGAAG | NCBI Reference Sequence: XM_039101034.1 |
| R-TCCAGTGTGTAGCGTGTGGAAATAG | ||
| HIF-1α | F-ACAAGTCACCACAGGACAG | NCBI Reference Sequence: NM_024359.2 |
| R-AGGGAGAAAATCAAGTCG | ||
| Dvl1 | F-TCACCGACTCTACCATGTCC | NCBI Reference Sequence: NM_031820.1 |
| R-ATACGATCTCCCGAAGCAC | ||
| GSK3β | F-CTGCCCTCTTCAACTTTACC | NCBI Reference Sequence: NM_032080.1 |
| R-TATTGGTCTGTCCACGGTCT | ||
| β-catenin | F-TCTAGTGCAGCTTCTGGGTT | NCBI Reference Sequence: NM_053357.2 |
| R-GATGGCAGGCTCGGTAATG |