Table 3.
Optical set up
| Laser source wavelength (nm) | Combining dichroic mirror from Chroma | Reflecting light range (nm) | Transmitting light range (nm) | Example of compatible fluorochromes |
|---|---|---|---|---|
| 405 | ZT405rdc | 392–415 | > 432 | TagBFP, Brilliant Violet 510, Pacific Blue, Brilliant Violet 570, Brilliant Violet 605 |
| 488 | ZT488rdc | 400–491 | > 498 | DiO, LysoTracker Yellow HCK-123, EGFP, GFP, Cy2, Emerald GFP, BB515, FITC, MitoTracker Green FM/MeOH, Oregon Green 488, Abberior rsEGFP, DyLight 488, FAM, mWasabi, Calcein, Fluo-4, Alexa Fluor 488, SYBR Green I |
| 561 | ZT561rdc | 430–564 | > 573 | Abberior Flip 565, CAL Fluor Red 590, Rhodamine Red-X |
| 637 | ZT640rdc | 495–644 | > 652 | Nile Blue, Abberior Star Red, Abberior Star 635, TO-PRO-3, Atto 647N, DiD, MitoTracker Deep Red 633/MeOH, Draq5, Quasar 670 |
Note: Dichroic mirrors were selected depending on the chosen laser sources. These optical components only reflect light in a defined range wavelength while allowing other wavelengths to pass through.