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. 2022 Feb 9;13:753570. doi: 10.3389/fimmu.2022.753570

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Copyright © 2022 Scott, Prabhakara, Walters, Olson and Cox

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Time points post-injury for each study. All rats underwent either sham or CCI injury. For cytokine studies of brain tissue, including individual ELISAs and a multiplex cytokine assay (performed using the LSR-II flow cytometer [BD Bioscience]), rats were sacrificed 6 hours after injury, to capture the peak of cytokine production. Rats were sacrificed 72 hours post-injury for our assessment of blood-brain barrier permeability. Flow cytometry and t-SNE of microglia and splenocyte immunophenotype using the Beckman-Coulter Gallios flow cytometer was performed 7 days after injury. Created with BioRender.com.