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Southern blot hybridization analysis of selected M. ulcerans strains probed with IS2404 (A) and IS2606 (B). Lane 1, strain 5142; lane 2, strain 13822/70; lanes 3 to 5, Chant strain. Genomic DNA was digested with PvuII (lanes 1 to 3), NcoI (lane 4), and EcoRI (lane 5); lane M, λ HindIII molecular size marker (Boehringer Mannheim).
