Bb strains differed in their ability to induce anti-spirochete antibodies in robin and white-footed (WF) mouse infection. (a–j) Sera were obtained at indicated days post nymph feeding (dpf). The levels of IgG against an equal ratio of B31-5A4, 297 and cN40 lysates were determined by ELISA. Data represents the geometric mean ± geometric s.d. Significance (p ≤ 0.05) from the uninfected group (*) or between groups (#) are indicated. (k,l) Sera from infected robins and WF mice at 7 dpf, or naive nymphs (uninfect.). Data represent the mean ± s.e.m. of the survival percentage. 50% BA, the dilution rate that killed 50% of spirochetes, was obtained from curve-fitting. N.F., not fittable. (Online version in colour.)