FIG. 1.
Southern and Western blot analyses to identify homologues of the M. pneumoniae 116-kDa protein in other Mycoplasma species. (a) Southern blot of genomic DNAs of species in the M. pneumoniae group, M. penetrans, and M. iowae (2, 25) digested with the enzymes indicated above the lanes and probed with oligonucleotide 116kcon. The sizes of the M. pneumoniae and M. genitalium genomic DNA fragments that hybridized with 116kcon are indicated to the left of the autoradiograph. Note that the M. pirum and M. iowae DNA were not digested by BglII (or BamHI), suggesting GATm5T methylation. (b) Whole-cell proteins from the Mycoplasma species indicated at the top were Western blotted and probed with immunoaffinity-purified rabbit antibodies to the 116-kDa protein. The position of the 116-kDa protein band is indicated to the right. The positions of New England BioLabs broad-range prestained protein markers are indicated to the left. These images were captured by using a Nikon Touchscan and Adobe Photoshop.