TABLE 2.
Fusion proteins spanning the 116-kDa protein
| Plasmid | Fusion protein | Amino acid range(s)a | Predicted molecular mass (kDa) |
|---|---|---|---|
| pGEX-3X-MP661 | 661 | 9–474 | 53 (79.6)c |
| pGEX-1N-MP3 | 3c | 467–709 | 27.4 (54) |
| pGEX-3X-MPFP2 | FP2 | 709–850 | 17 (43.7) |
| pGEX-1N-MP10 | 10c | 846–896 | 5.7 (32.3) |
| pGEX-3X-MPFP3 | FP3 | 887–962, 887–967, 887–1029b | 9.2 (35.8) |
| pGEX-3X-MPFP4 | FP4 | 969–1029 | 7.4 (34) |
a
The positions of the first and last amino acids of the 116-kDa protein contained in each fusion protein are shown.
b
The PCR product generated by FP3F and FP3R spanned two TGA stop codons (TGA codes for Trp in mycoplasma). Consequently, three fusion proteins were expressed from plasmid pGEX-3X-MPFP3, but most of the fusion proteins terminated at the first TGA (encoding Trp amino acid 963).
c
Each value in parentheses is the molecular mass of the protein fused to GST.