PPARα, PPARγ, and CB1 antagonists did not block the inhibitory effect of WIN on the IL1β-induced inflammatory gene transcription. (A) Fold-change of IL6 mRNA transcript levels normalized to ACTB mRNA levels in total RNA isolated from human astrocytes. One-way ANOVA was conducted [F (12, 13)=55.96, p<0.0001]. (B) Fold-change of C3 mRNA transcript levels normalized to ACTB mRNA levels in total RNA isolated from human astrocytes. One-way ANOVA was conducted [F (12, 13)=90.84, p<0.0001]. A post hoc Tukey's test was conducted; corrected p-values are shown (***p<0.001, ****p<0.0001 vs. vehicle; n.s. vs. WIN+IL1β). CB1, cannabinoid receptor 1; PPAR, peroxisome proliferator-activated receptors.