REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
chicken anti-GFP	Abcam plc.	RRID: AB_300798
mouse IgG1 anti-3A10	DSHB	RRID: AB_531874
rabbit anti-Neurofascin	Abcam plc.	RRID: AB_2905463
goat anti-mouse IgG, Alexa Fluor 633	Thermo Fisher	RRID: AB_2535719
goat anti-rabbit IgG, Alexa Fluor 555	Thermo Fisher	RRID: AB_141784
goat anti-chicken IgY, Alexa Fluor 488	Thermo Fisher	RRID: AB_142924
Experimental models: Organisms/strains
Tg(mbp:memCerulean)tum101Tg	Auer et al. (2018)	ZFIN: ZDB-ALT-180712-3
Tg(mbp:KillerRed)tum103Tg	Auer et al. (2018)	ZFIN: ZDB-ALT-180712-5
Tg(mbp:tagRFPt-CAAX)tum102Tg	Auer et al. (2018)	ZFIN: ZDB-ALT-180712-4
Tg(cntn1b(3kb):KalTA4)	This paper	N/A
Tg(olig1:mScarlet-CAAX)	This paper	N/A
CRISPR-NfascaΔ28	This paper	N/A
Oligonucleotides
For all oligonucleotides used, see Table S1	this paper	N/A
Recombinant DNA
p5E_cntn1b(3kb)	this paper	N/A
pME_EYFPnostop_NaV-II-III	this paper	N/A
pME_sigpepEYFP_cntn1a	this paper	N/A
p5E_cntn1b(5kb)	Czopka et al. (2013)	N/A
pME_KalTA4GI	Almeida and Lyons (2015)	N/A
p5E_UAS(10x)	Kwan et al. (2007)	N/A
p3E_pA	Kwan et al. (2007)	N/A
pDestTol2_pA	Kwan et al. (2007)	N/A
pME_tagCFP	Auer et al. (2018)	N/A
pTol2_cntn1b(5kb):Nfasca-EYFP	Auer et al. (2018)	N/A
pTol2_cntn1b(5kb):EYFP-cntn1a	this paper	N/A
pTol2_cntn1b(5kb):EYFP-NaV-II-III	this paper	N/A
pTol2_UAS(10x):EYFP-cntn1a	this paper	N/A
pTol2_cntn1b(5kb):tagCFP	this paper	N/A
pTol2_cntn1b(5kb):mCherry	Czopka et al. (2013)	N/A
Software and algorithms
Fiji	http://fiji.sc/	RRID: SCR_002285
Imaris	Bitplane	RRID: SCR_007370
Imaris FilamentTracer	Bitplane	RRID: SCR_007366
Huygens Essential	Scientific Volume Imaging	RRID: SCR_014237
Graphpad Prism 8	Graphpad Software	RRID: SCR_015807
Code for simulation of cluster-node correlation	This paper	ZENODO DOI: https://doi.org/10.5281/zenodo.5846391.