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. 2022 Feb 15;38(7):110377. doi: 10.1016/j.celrep.2022.110377

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© 2022 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Patterns of islet cell emergence during embryonic development

(A) Graphical design of the experiment. Embryonic hormone-expressing cells are labeled from E7.5 for different duration.

(B) Quantification of the YFP-traced Gcg⁺ cells at postnatal day 30 (P30). No DOX: n = 4, E7.5 to E12.5: n = 6, E7.5 to E13.5: n = 4, E7.5 to E14.5: n = 5, E7.5 to E15.5: n = 5, E7.5 to E16.5: n = 5, E7.5 to E17.5: n = 7, E7.5 to E17.5: n = 5, E7.5 to E20.5: n = 9. p values: No DOX versus E7.5 to E12.5 = 0.0381; E7.5 to E14.5 versus E7.5 to E15.5 = 0.0079; E7.5 to E17.5 versus E7.5 to E18.5 = 0.0303. Representative pictures of the Gcg-expressing YFP-labeled cells when DOX is given from E7.5 to E13.5 (left), to E16.5 (middle), or to E20.5 (right). Red, Gcg; Green, YFP. Scale bars, 20μm.

(C) Quantification of the YFP-traced Ins⁺ cells at P30. No DOX: n = 4, E7.5 to E12.5: n = 10, E7.5 to E13.5: n = 5, E7.5 to E14.5: n = 5, E7.5 to E15.5: n = 4, E7.5 to E16.5: n = 6, E7.5 to E18.5: n = 4, E7.5 to E20.5: n = 6. p values: No DOX versus E7.5 to E12.5 = 0.009; E7.5 to E12.5 versus E7.5 to E13.5 = 0.001; E7.5 to E14.5 versus E7.5 to E15.5 = 0.0159; E7.5 to E16.5 versus E7.5 to E17.5 = 0.0043. Representative pictures of the Ins-expressing YFP-labeled cells when DOX is given from E7.5 to E13.5 (left), to E16.5 (middle), or to E20.5 (right). Red, Ins; Green, YFP. Scale bars, 20μm.

(D) Quantification of the YFP-traced Sst⁺ cells at P30. No DOX: n = 4, E7.5 to E12.5: n = 5, E7.5 to E13.5: n = 5, E7.5 to E14.5: n = 11, E7.5 to E15.5: n = 6, E7.5 to E16.5: n = 16, E7.5 to E17.5: n = 9, E7.5 to E18.5: n = 3, E7.5 to E20.5: n = 4. p values: E7.5 to E13.5 versus E7.5 to E14.5 = 0.0034; E7.5 to E16.5 versus E7.5 to E17.5 = 0.0001. Representative pictures of the Sst-expressing YFP-labeled cells when DOX is given from E7.5 to E13.5 (left), to E16.5 (middle), or to E20.5 (right). Red, Sst; Green, YFP. Scale bars, 20μm.

(E) Quantification of the YFP-traced Ppy⁺ cells at P30. No DOX: n = 4, E7.5 to E12.5: n = 6, E7.5 to E13.5: n = 8, E7.5 to E14.5: n = 9, E7.5 to E16.5: n = 4, E7.5 to E17.5: n = 4, E7.5 to E18.5: n = 6, E7.5 to E20.5: n = 4. p values: no DOX versus E7.5 to E12.5 = 0.0095; E7.5 to 12.5 versus E7.5 to 13.5 = 0.0007; E7.5 to 15.5 versus E7.5 to 16.5 = 0.0424; E7.5 to 17.5 versus E7.5 to 18.5 = 0.0095; E7.5 to 18.5 versus E7.5 to 20.5 = 0.0012. Representative pictures of the Ppy-expressing YFP-labeled cells when DOX is given from E7.5 to E13.5 (left), to E16.5 (middle), or to E20.5 (right). Red, Ppy; Green, YFP. Scale bars, 20μm. Data are shown as mean ± SEM; two-tailed Mann-Whitney test. Gcg⁺, Sst⁺, and Ins⁺ cells were scored in sections from dorsal pancreata, while Ppy⁺ cells were scored in the ventral pancreas. One representative biological replicate of an experiment is presented in the micrographs. Experiments were performed two or more times independently under identical or similar conditions. Quantification details are provided as Data S1E.