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. 2022 Feb 15;38(7):110377. doi: 10.1016/j.celrep.2022.110377

Figure 3.

Figure 3

No evidence of postnatal persistence of islet precursor cells

(A) Graphical design of the experimental rationale.

(B) Labeling strategy of embryonic hormone-expressing cells.

(C) The proportion of adult YFP-labeled Gcg-expressing cells is maintained in the different DOX administration periods. DOX E7.5 to P30, analysis P30 (black; n = 4); DOX E7.5 to E20.5, analysis P30 (dark gray, n = 9); DOX E7.5 to E20.5, analysis 10-month-old (light gray, n = 6). p values: DOX E7.5 to P30, analysis P30 versus DOX E7.5 to E20.5, analysis P30 = 0.6042; DOX E7.5 to P30, analysis P30 versus DOX E7.5 to E20.5, analysis long term = 0.271.

(D) The proportion of adult YFP-labeled Ins-expressing cells is maintained in the different DOX administration periods. DOX E7.5 to P30, analysis P30 (black; n = 6); DOX E7.5 to E20.5, analysis P30 (dark gray, n = 5); DOX E7.5 to E20.5, analysis 10-month-old (light gray, n = 4). p values: DOX E7.5 to P30, analysis P30 versus DOX E7.5 to E20.5, analysis P30 = 0.6277; DOX E7.5 to P30, analysis P30 versus DOX E7.5 to E20.5, analysis long term = 0.719.

(E) The proportion of adult YFP-labeled Sst-expressing cells is maintained in the different DOX administration periods. DOX E7.5 to P30, analysis P30 (black; n = 5); DOX E7.5 to E20.5, analysis P30 (dark gray, n = 4); DOX E7.5 to E20.5, analysis 10-month-old (light gray, n = 3). p values: DOX E7.5 to P30, analysis P30 versus DOX E7.5 to E20.5, analysis P30 = 0.2857; DOX E7.5 to P30, analysis P30 versus DOX E7.5 to E20.5, analysis long term = 0.8571. Data are shown as mean ± SEM; two-tailed Mann-Whitney test. Region of the pancreas: Gcg+ and Ins+ cells were scored in the dorsal pancreas, Sst+ and Ppy+ cells were scored in the ventral pancreas. Experiments were performed two or more times independently under identical or similar conditions. Quantification details are provided as Data S1F.