Figure 4.

Embryonic hormone-expressing cells have variable fates in adult islets

(A) Cartoon depicting the experimental rationale.

(B) Strategy for labeling embryonic hormone-expressing cells.

(C) Proportion of adult hormone-expressing cells labeled with YFP using the Gcg-YFPi tracing system. p values: Ins⁺ cells (E7.5 to E20.5 versus P0 to P30) = 0.0025; Sst⁺ cells (E7.5 to E20.5 versus P0 to P30) = 0.0016.

(D) Proportion of adult hormone-expressing cells labeled with YFP using the Mip-YFPi tracing system. p values: Gcg⁺ cells (E7.5 to E20.5 versus P0 to P30) = 0.1558; Ins⁺ cells (E7.5 to E20.5 versus P0 to P30) = 0.9307; Sst⁺ cells (E7.5 to E20.5 versus P0 to P30) = 0.9134; Ppy+ cells (E7.5 to E20.5 versus P0 to P30) = 0.2251.

(E) Proportion of adult hormone-expressing cells labeled with YFP using the Sst-YFPi tracing system. p values: Ins⁺ cells (E7.5 to E20.5 versus P0 to P30) = 0.0079; Ppy⁺ cells (E7.5 to E20.5 versus P0 to P30) = 0.001. Data are shown as mean ± SEM; two-tailed Mann-Whitney test.

(F) Immunofluorescence of adult YFP-labeled Ins- (top left, red rectangle; Ins, red; YFP, green; Gcg, gray) and Ppy- (top middle, green rectangle; Ppy, red; YFP, green; Gcg, gray) expressing cells after DOX administration during the embryonic period using the Gcg-YFPi system. Using the Sst-YFPi system, some adult Ppy-expressing cells are YFP-labeled (top right, blue rectangle; Ppy, red; YFP, green; Sst, gray). Bihormonal YFP-labeled Ins⁺ Gcg⁺ (bottom left; Ins, red; YFP, green; Gcg, gray), Gcg⁺ Ppy⁺ (bottom middle; Ppy, red; YFP, green; Gcg, gray) and Sst⁺ Ppy⁺ (bottom right; Ppy, red; YFP, green; Sst, gray) cells were detected at E17.5. Scale bars, 20μm (10μm in insets). One representative biological replicate of an experiment is presented in the micrographs. Experiments were performed two or more times independently under identical or similar conditions. Quantification details are provided as Data S1G.