REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-insulin	Molecular Probes	Cat# 701265; RRID :AB_2532448
Mouse anti-glucagon	Sigma	Cat# G2654; RRID: AB_259852
rabbit anti-somatostatin	DAKO	Cat# A0566; RRID: AB_2688022
mouse anti-Ppy (clone 23-2D3)	IBL	Cat# 10501
rabbit anti-GFP	Molecular Probes	Cat# A11122; RRID: AB_221569
Chemicals, peptides, and recombinant proteins
Doxycycline (1 mg/ml)	Sigma	Catalog # D9891
PowerUp™ SYBR™ Green Master Mix	ThermoFisher	Catalog # A25742
Histopaque-1119	Sigma	Catalog # 11191
Collagenase IV	Sigma	Catalog # C7657
guinea pig anti-porcine insulin (1/400)	Dako	Cat# A0564; RRID: AB_10013624
rabbit anti-insulin (1/3000)	Molecular Probes	Cat# 701265; RRID :AB_2532448
mouse anti-glucagon (1/1000)	Sigma	Cat# G2654; RRID: AB_259852
rabbit anti-glucagon (1/200)	Dako	Cat# A0565; RRID: AB_10013726
mouse anti-somatostatin (1/200)	BCBC	Cat# Ab1985; RRID: AB_10014609
rabbit anti-somatostatin (1/200)	Dako	Cat# A0566; RRID: AB_2688022
goat anti-somatostatin (1/200)	Santa Cruz Biotechnology	Cat# sc-55565; RRID: AB_831726 (out of stock)
rabbit anti-GFP (1/400)	Molecular Probes	Cat# A11122; RRID: AB_221569
chicken anti-GFP (1/500)	Abcam	Cat# ab13970; RRID: AB_300798
mouse anti-Ppy (clone 23-2D3)	IBL	Cat# 10501
mouse anti-Ppy (1/1000)	R&D Biosystems	Cat# MAB62971; RRID: AB_11127208
Alexa 568, 488, 647 and 405 Secondary Ab	Molecular Probes	N/A
Cy3, Cy5, FITC and TRITC Secondary Ab	Southern Biotech	N/A
Critical commercial assays
Qiagen RNeasy Micro Kit	Qiagen	Catalog # 74004
Qiagen QuantiTect Reverse Transcription Kit	Qiagen	Catalog # 205311
Experimental models: Organisms/strains
Mouse: Glucagon Promoter-rtTA;TetO-Cre;R26-YFP	Thorel et al., 2010	https://doi.org/10.1038/nature08894
Mouse: Somatostatin Promoter-rtTA;TetO-Cre;R26-YFP	Cigliola et al., 2018	https://doi.org/10.1038/s41556-018-0216-y
Mouse: Mouse Insulin promoter-rtTA;TetO-Cre;R26-YFP	Kusminski et al., 2016	https://doi.org/10.2337/db15-1323
Mouse: Pancreatic Polypeptide promoter-rtTA;TetO-Cre;R26-YFP	Perez-Frances et al., 2021	https://doi.org/10.1038/s41467-021-24788-0
Oligonucleotides
qPCR primers for hormones and Cre recombinase, See Table S2	This paper	N/A
Software and algorithms
Prism v8.0.1 software	N/A	https://www.graphpad.com/scientific-software/prism/
Leica Application Suite X 3.0.2.16120	Leica Microsystems	https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/