Table 1.

Sera for MMACA assay development and characterization.

Reagent ID	Formulation Dilution Factor	Expected IgG concentration (IU or AU/mL)
Reagent ID	Formulation Dilution Factor	Act	Fha	Fim2/3	Prn	Pt

2012812612^a	Neat	129.92	113.58	1153.47	1299.40	171.28
MCR0001	2	64.96	56.79	576.74	649.70	85.64
MCR0002	4	32.48	28.40	288.37	324.85	42.82
MCR0003	16	8.12	7.10	72.09	81.21	10.71
MCR0004	30	4.33	3.79	38.45	43.31	5.71
MCR0005	120	1.08	0.95	9.61	10.83	1.43
MCR0006	500	0.26	0.23	2.31	2.60	0.34
MCR0007	2000	0.06	0.06	0.58	0.65	0.09

2012789123^a	Neat	264.62	1015.55	320.60	1771.89	45.86
MCR0008	2	132.31	507.78	160.30	885.95	22.93
MCR0009	4	66.16	253.89	80.15	442.97	11.47
MCR0010	16	16.54	63.47	20.04	110.74	2.87
MCR0011	30	8.82	33.85	10.69	59.06	1.53
MCR0012	120	2.21	8.46	2.67	14.77	0.38
MCR0013	500	0.53	2.03	0.64	3.54	0.09
MCR0014	2000	0.13	0.51	0.16	0.89	0.02

WHO06/142^a	Neat	64.65	107.11	38.51	29.50	82.88
MCR0015	2	32.33	53.56	19.26	14.75	41.44
MCR0016	4	16.16	26.78	9.63	7.38	20.72
MCR0017	6	10.78	17.85	6.42	4.92	13.81
MCR0018	12	5.39	8.93	3.21	2.46	6.91
MCR0019	50	1.29	2.14	0.77	0.59	1.66
MCR0020	200	0.32	0.54	0.19	0.15	0.41
MCR0021	750	0.09	0.14	0.05	0.04	0.11

Act – Adenylate cyclase; Fha – filamentous hemagglutinin; Fim2/3 - Fimbria; Prn – Pertactin; Pt – Pertussis toxin antigens.

Human sera spiked into human serum depleted of total IgG, IgA and IgM (MCR0028) to formulate a serum panel (MCR0001 – MCR0021) for MMACA assay development and characterization.