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(Left) Kernel density estimate of mass shifts around +1 Da obtained from 24 LC-MS files of Proteome Exchange data set PXD001468. A small bulge near shift of +0.98 Da can be discerned, corresponding to deamidation, but the shape of KDE is very noisy overall. (Middle) Zero-peak correction applied. The KDE now looks like a mixture of two Normal distributions. (Right) Mass recalibration using identified peptides applied to the same data set.
