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. 2022 Feb 11;25(4):123. doi: 10.3892/mmr.2022.12639

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Copyright: © Liang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 7. — Knockdown of DDX11-AS1 upregulates the expression levels of miR-497. (A) RT-qPCR assay was used to detect the expression levels of miR-497 in breast cancer cell lines. ***P<0.001 vs. MCF-10A. (B) Luciferase reporter gene assay was used to verify the relationship between DDX11-AS1 and miR-497. ***P<0.001 vs. Vector. RT-qPCR assay detected the expression levels of miR-497 in (C) MCF-7/PTX and (D) MDA-MB-231/PTX cells after transfection. ***P<0.001 vs. shRNA-NC. DDX11-AS1, DDX11 antisense 1; miR-497, microRNA-497; NC, negative control; RT-qPCR, reverse transcription-quantitative PCR; shRNA, short hairpin RNA.