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. 2022 Feb 14;25(4):124. doi: 10.3892/mmr.2022.12640

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Copyright: © Huang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Restoration of JPH3 expression by treatment with 5-Aza demethylation inhibits proliferation, migration and invasion in HCC cells. (A) Transwell assays were used to analyze the effect of restoration of JPH3 expression by treatment with 5-Aza-2′-deoxycytidine (5-Aza) on the invasion of Hep3B cells (magnification, ×200). (B) Transwell assays were used to analyze the effect of restoration of JPH3 expression by treatment with 5-Aza on the invasion of Huh-7 cells (magnification, ×200). (C) A wound healing assay was used to analyze the effect of restoration of JPH3 expression by treatment with 5-Aza on the migration of Hep3B cells (magnification, ×100). (D) A wound healing assay was used to analyze the effect of restoration of JPH3 expression by treatment with 5-Aza on the migration of Huh-7 cells (magnification, ×100). (E) Colony formation experiments were also used to analyze the restoration of JPH3 expression by treatment with 5-Aza on the proliferation of Hep3B cells. (F) Colony formation experiments were also used to analyze the restoration of JPH3 expression by treatment with 5-Aza on the proliferation of Huh-7 cells. *P<0.05. HCC, hepatocellular carcinoma; JPH3, junctophilin 3.