Figure 1.

Alterations in the composition of the colonic microbial community in mice with a selective Atg5-deficiency in myeloid cells. Bacterial composition as assessed by 16S rRNA sequencing of DNA extracted from freshly collected stool of Atg5-Wt and Atg5ΔMye mice [n = 5 per group]. [A] Pie chart showing the average proportion of Firmicutes, Bacteroidetes, Actinobacteria, Proteobacteria, and all other phyla in 8-week old mice. [B‐E] Quantification of the phyla [B] Firmicutes, [C] Bacteroidetes, [D] Actinobacteria, and [E] Proteobacteria. [F] Heatmap of the relative abundance of colonic microbes [genus level]. [G] Principal coordinates analysis [PCoA] plot of microbiota composition [genus level]. Data are shown as mean (±95% confidence interval [CI]) and a t test was used to measure specific microbiome species abundance between groups. Adjusted p-value >0.05 was used as significant threshold.