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. 2021 Aug 10;16(2):259–274. doi: 10.1093/ecco-jcc/jjab144

Figure 4.

Figure 4.

Atg5 regulates IL-12 secretion in macrophages. Analysis of IL-12 secretion in macrophages. [A] Detection of IL-12 by ELISA from Atg5-Wt or Atg5ΔMye BMM after LPS and IFNγ stimulation. [B and C] Detection of IL-12 by ELISA from Atg5-Wt [B] or Atg5ΔMye [C] BMM after LPS and IFNγ stimulation in the presence or absence of Baf. A1. Representative of two independent of experiments. [D‐F] 1–3 µm Z-stack images were performed on BMM stimulated with LPS, IFNγ, and Baf. A1 using immunofluorescence at 1.6 Zoom by a 63x oil immersion objective. [D] Atg5-Wt BMM were stained for LAMP1 [green] and IL-12p35 [red]. [E] Atg5-Wt BMM were stained for ATG5 [red] and IL-12p35 [green]. [F] Atg5ΔMye BMM were stained for LAMP1 [green] and IL-12p35 [red]. Representative from 65 images from 15 slides from two independent of experiments. Arrows in images indicate puncta co-localising with insets displaying enlargement of indicated region. Scale bars: 10 µm. Graphs indicate mean [±SD]; *p <0.05, **p <0.01,***p <0.001. Two-tailed unpaired Student’s t tests. ELISA, enzyme-linked immunosorbent assay; BMM, bone marrow-derived macrophages; LPS, lipopolysaccharide; SD,standard deviation.